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Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Immunocytochemistry of TLR8 in Daudi cells with TLR8 antibody at 2 μg/mL.
Immunofluorescence of TLR8 in Daudi cells with TLR8 antibody at 10 μg/mL.
Western blot analysis of TLR8 in Daudi cell lysates with TLR8 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
Trial Size

TLR8 Antibody

CATALOG NUMBER: 3281

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TLR8 antibody was raised against a peptide corresponding to 16 amino acids near the middle of human TLR8.
The immunogen is located within amino acids 790 - 840 of TLR8.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TLR8 antibody can be used for detection of TLR8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1224 - Daudi Cell Lysate
Predicted Molecular Weight:
Predicted: 112 kDa
Observed: 107 kDa
Purification:
TLR8 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TLR8 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TLR8 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TLR8
Additional Names:
TLR8 Antibody: CD288, Toll-like receptor 8
Protein Accession Number:
NP_619542
Protein Gi Number:
20302168
Ncbi Gene Id Number:
51311
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TLR8 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. Like TLR7, TLR8 is localized to endosomal or lysosomal compartments and stimulates the innate immune response after activation by guanosine- and uridine-rich single-stranded RNA. Human but not murine TLR8 confers responsiveness to the antiviral compound R-848.
Background Reference 1:
Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77.
Background Reference 2:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 3:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216.
Background Reference 4:
O'Neill LAJ, Fitzgerald FA, and Bowie AG. The Toll-IL-1 receptor adaptor family grows to five members. Trends in Imm. 2003; 24:286-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3281

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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