Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TMEM135 in rat liver tissue lysate with TMEM135 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TMEM135 in human liver tissue with TMEM135 antibody at 2.5 μg/ml.
Immunofluorescence of TMEM135 in human liver tissue with TMEM135 antibody at 20 μg/ml.
Western blot analysis of TMEM135 in rat liver tissue lysate with TMEM135 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TMEM135 Antibody

CATALOG NUMBER: 7291

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
TMEM135 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human TMEM135 .
The immunogen is located within the last 50 amino acids of TMEM135.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TMEM135 Antibody can be used for detection of TMEM135 by Western blot starting at 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of TMEM135 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 50 kDa
Observed: 53 kDa
Purification:
TMEM135 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM135 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM135 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TMEM135
Additional Names:
TMEM135 Antibody: PMP52, Transmembrane protein 135, Peroxisomal membrane protein 52, PMP52
Protein Accession Number:
NP_075069
Protein Gi Number:
281182571
Ncbi Gene Id Number:
65084
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM135 Antibody: The transmembrane protein 135 (TMEM135), also known as peroxisomal membrane protein 52 (PMP52), was initially identified as a protein that might be critical for adipogenesis and osteoblastogenesis. Further studies have indicated that TMEM135 may be part of a regulatory circuit that plays an important role in fat metabolism and energy expenditure in both C. elegans as well as mammalian organisms. Finally, recent experiments has suggested that TMEM135 may be an additional driver of breast cancer.
Background Reference 1:
Scheideler M, Elabd C, Zaragosi LE, et al. Comparative transcriptomics of human multipotent stem cells during adipogenesis and osteoblastogenesis. BMC Genomics 2008; 9:340.
Background Reference 2:
Exil VJ, Avila DS, Benedetto A, et al. Stress-induced TMEM135 protein is part of a conserved genetic network involved in fat storage and longetivity regulation in Caenorhabditis elegans. PLoS One 2010; 5:e14228.
Background Reference 3:
Natrajan R, Mackay A, Lambros MB, et al. A whole-genome massively parallel sequencing analysis of BRCA1 mutant oestrogen receptor-negative and –positive breast cancers. J. Pathol. 2012; 227:29-41.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7291

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369