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Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/mL.
Western blot analysis of TMEM16A in A549 cell lysate with TMEM16A antibody at 1 μg/mL.
Trial Size

TMEM16A Antibody

CATALOG NUMBER: 5419

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TMEM16A antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human TMEM16A.
The immunogen is located within amino acids 70 - 120 of TMEM16A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TMEM16A antibody can be used for detection of TMEM16A by Western blot at 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
TMEM16A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM16A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM16A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANO1
Additional Names:
TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1
Protein Accession Number:
NP_060513
Protein Gi Number:
194306539
Ncbi Gene Id Number:
55107
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM16A Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16A is expressed in epithelial cells of the kidney and lung, pancreas, and sensory neurons and its mRNA is seen in foregut, airway epithelia, and tracheal smooth muscle; mice lacking TMEM16A fail to survive past ten days and show aerophagia and little weight gain. TMEM16A is also overexpressed or amplified in multiple cancers associated with poor survival such as oral cancers and gastrointestinal stromal tumors, suggesting the development of CaCC modulators may be a viable therapeutic strategy.
Background Reference 1:
Schroeder BC, Cheng T, Jan YN, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell2008; 134:1019-29.
Background Reference 2:
Rock JR, Futtner CR, and Harfe BD. The transmembrane protein TMEM16A is required for normal development of the murine trachea. Dev. Biol.2008; 214:399-411.
Background Reference 3:
Huang X, Godfrey TE, Gooding WE, et al. Comprehensive genome and transcriptome analysis of the 11q13 amplicon in human oral cancer and synteny to the 7F5 amplicon in murine oral carcinoma. Genes Chromosomes Cancer2006; 45:10458-69.
Background Reference 4:
West RB, Corless CL, Chen X, et al. The novel marker, DOG1, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of KIT or PDGRA mutation status. Am. J. Pathol.2004; 165:107-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5419

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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