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Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TMEM16B in human brain tissue with FRMPD4 antibody at 5 μg/mL.
Immunofluorescence of TMEM16B in Human Brain cells with TMEM16B antibody at 20 μg/mL.
Western blot analysis of TMEM16B in human small intestine tissue lysate with TMEM16B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TMEM16B Antibody

CATALOG NUMBER: 5421

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TMEM16B antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human TMEM16B.
The immunogen is located within the first 50 amino acids of TMEM16B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TMEM16B antibody can be used for detection of TMEM16B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 110 kDa
Observed: 107 kDa
Purification:
TMEM16B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM16B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM16B antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ANO2
Additional Names:
TMEM16B Antibody: C12orf3, TMEM16B, C12orf3, Anoctamin-2, Transmembrane protein 16B
Protein Accession Number:
NP_001265525
Protein Gi Number:
209862785
Ncbi Gene Id Number:
57101
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM16B Antibody: Calcium-activated chloride channels (CaCC) are present in many cell types and mediate physiological functions such as epithelial secretion, sensory signal transduction, and smooth muscle contraction. Subunits of these CaCC's include the transmembrane proteins TMEM16A and TMEM16B. TMEM16B is predicted to have eight transmembrane domains with both the amino and carboxy termini in the cytoplasm and is expressed in several tissues including olfactory sensory neurons as well as photoreceptors in mammalian retina. Like TMEM16A, TMEM16B is thought to form at least part of CaCC's but has different biophysical characteristics such as voltage dependence and unitary conductance.
Background Reference 1:
Schroeder BC, Cheng T, Jan YN, et al. Expression cloning of TMEM16A as a calcium-activated chloride channel subunit. Cell2008; 134:1019-29.
Background Reference 2:
Stephan AB, Shum EY, Hirsh S, et al. ANO2 is the cilial calcium-activated chloride channel that may mediate olfactory amplification. Proc. Natl. Acad. Sci.USA 2009; 106:11776-81.
Background Reference 3:
Pifferi S, Dibattista M, and Menini A. TMEM16 induces chloride currents activated by calcium in mammalian cells. Pflugers Arch.2009; 458:1023-38.
Background Reference 4:
Stohr H, Heisig JB, Benz PM, et al. TMEM16B, a novel protein with calcium-dependent chloride channel activity, associates with a presynaptic protein complex in photoreceptor terminals. J. Neurosci.2009; 29:6809-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5421

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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