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Western blot analysis of TMEM204 in human brain tissue lysate with TMEM204 antibody at 1 μg/mL .
Immunohistochemistry of TMEM204 in mouse brain tissue with TMEM204 antibody at 5 μg/mL.
Immunofluorescence of TMEM204 in mouse brain tissue with TMEM204 antibody at 20 μg/mL.
Western blot analysis of TMEM204 in human brain tissue lysate with TMEM204 antibody at 1 μg/mL .
Trial Size

TMEM204 Antibody

CATALOG NUMBER: 6415

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TMEM204 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TMEM204.
The immunogen is located within the last 50 amino acids of TMEM204.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TMEM204 antibody can be used for detection of TMEM204 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of TMEM204 are known to exist; this antibody will recognize both isoforms.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
TMEM204 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM204 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM204 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMEM204
Additional Names:
TMEM204 Antibody: CLP24, C16orf30, CLP24, UNQ6509/PRO21434, Transmembrane protein 204, Claudin-like protein 24
Protein Accession Number:
NP_078876
Protein Gi Number:
112807182
Ncbi Gene Id Number:
79652
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM204 Antibody: Transmembrane protein 204 (TMEM204) is a four-transmembrane protein that is induced under hypoxic conditions. It localizes to cell-cell junctions and co-localizes with the beta-catenin adherens junction-associated protein, but not with tight junctions. Overexpression of TMEM204 results in decreased adhesion between cells, indicating that TMEM204 is an adherens junction protein that modulates cell adhesion and potentially angiogenesis. Recent evidence suggests TMEM204 may also be involved in epilepsy.
Background Reference 1:
Kearsey J, Petit S, De Oliveira C, et al. A novel four transmembrane spanning protein, CLP24. Eur. J. Biochem. 204; 271:2584-92.
Background Reference 2:
Stefaniuk M and Lukasiuk K. Cloning of expressed sequence tags (ESTs) representing putative epileptogenesis-related genes and the localization of their expression in the normal brain. Neurosci. Lett. 2010; 482:230-4

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6415

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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