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Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of TMEM214 in A20 cells with TMEM214 antibody at 5 μg/mL.
Immunofluorescence of TMEM214 in A20 cells with TMEM214 antibody at 20 μg/mL.
Western blot analysis of TMEM214 in A20 cell lysate with TMEM214 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TMEM214 Antibody

CATALOG NUMBER: 5297

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
TMEM214 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TMEM214.
The immunogen is located within amino acids 70 - 120 of TMEM214.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TMEM214 antibody can be used for detection of TMEM214 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
TMEM214 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM214 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM214 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMEM214
Additional Names:
TMEM214 Antibody: Transmembrane protein 214
Protein Accession Number:
NP_060197
Protein Gi Number:
134152721
Ncbi Gene Id Number:
54867
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM214 Antibody: Transmembrane protein 214 (TMEM214) is a 77kD membrane protein which is widely expressed at high level. The gene encoding this protein maps to chromosome 2, at 2p23.3 TMEM214 interacts with LSM1, an SM-like protein, forming a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing. The detailed function of TMEM214 is still unknown, but a recent study showed that TMEM214 may be used to explore the contribution of human host factors of some infectious diseases such as Dengue fever, West Nile fever and yellow fever. Multiple isoforms of TMEM214 are known to exist.
Background Reference 1:
Wan D, Gong Y, Qin W, et al. Large-scale cDNA transfection screening for genes related to cancer development and progression. Proc. Natl. Acad. Sci. U S A2004;101:15724-9.
Background Reference 2:
Lehner B and Sanderson CM. A protein interaction framework for human mRNA degradation. Genome Res.2004; 14:1315-23.
Background Reference 3:
Sessions OM, Barrows NJ, Souza-Neto JA, et al. Discovery of insect and human dengue virus host factors. Nature2009; 458:1047-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5297

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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