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<strong>Figure 1 Western Blot Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysates per lane.Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 2 Western Blot Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
<strong>Figure 3 Immunofluorescence Validation of TMEM41B in HeLa Cells</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 4 Immunofluorescence Validation of TMEM41B in Human Lung</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 5 Immunofluorescence Validation of TMEM41B in Mouse Kidney </strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse kidney labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
<strong>Figure 6 Immunofluorescence Validation of TMEM41B in Rat Lung</strong><br>Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling TMEM41B with 9565 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 Western Blot Validation in Mouse Tissues
Loading: 15 μg of lysates per lane.Antibodies: TMEM41B 9565, 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
New
Trial Size

TMEM41B (CT) Antibody

CATALOG NUMBER: 9565

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine (100%); Chicken (100%); Chimpanzee (100%).
Immunogen:
Anti-TMEM41B antibody (9565) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human TMEM41B.
The immunogen is located within the last 50 amino acids of TMEM41B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
WB: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 – Mouse Kidney Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 33kD
Observed: 33 kD
Purification:
TMEM41B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM41B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM41B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMEM41B
Additional Names:
TMEM41B Antibody: Transmembrane protein 41B, Protein stasimon, TMEM41B, KIAA0033.
Protein Accession Number:
Q5BJD5
Protein Gi Number:
74741383
Ncbi Gene Id Number:
440026
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM41B Antibody: TMEM41B is required for autophagosome formation and participates in early stages of autophagosome biogenesis at the ER membrane probably via mobilization of neutral lipids from lipid droplets. TMRM41B is a critical host factor required for infection by human coronaviruses SARS-CoV-2, HCoV-OC43, HCoV-NL63, and HCoV-229E, as well as all flaviviruses tested such as Zika virus and Yellow fever virus. It is required post-entry of the virus to facilitate the ER membrane remodeling necessary to form replication organelles.
Background Reference 1:
Moretti et al. EMBO Rep. 2018; 19(9):e45889.
Background Reference 2:
Shoemaker et al. PLoS Biol. 2019; 17(4):e2007044.
Background Reference 3:
Schneider et al. Cell 2021; 184(1):120-132.
Background Reference 4:
Hoffmann et al. Cell 2021; 184(1):133-148.e20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9565

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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