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Western blot analysis of TMEM59 in human kidney tissue lysate with TMEM59 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TMEM59 in human kidney tissue with TMEM59 antibody at 2.5 μg/mL.
Immunofluorescence of TMEM59 in human kidney tissue with TMEM59 antibody at 20 μg/mL.
Western blot analysis of TMEM59 in human kidney tissue lysate with TMEM59 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TMEM59 Antibody

CATALOG NUMBER: 6063

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%), Mouse: (94%)
Immunogen:
TMEM59 antibody was raised against a 16 amino acid synthetic peptide near amino terminus of human TMEM59.
The immunogen is located within amino acids 80 - 130 of TMEM59.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TMEM59 antibody can be used for detection of TMEM59 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
TMEM59 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMEM59 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMEM59 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TMEM59
Additional Names:
TMEM59 Antibody: C1orf8, HSPC001, C1orf8, UNQ169/PRO195, Transmembrane protein 59, Liver membrane-bound protein
Protein Accession Number:
NP_004863
Protein Gi Number:
20070191
Ncbi Gene Id Number:
9528
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMEM59 Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59, a Golgi-localized protein, modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP. It inhibits APP transport and shedding.
Background Reference 1:
Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci.2006; 24:141-8.
Background Reference 2:
Schöbel S, Neumann S, Hertweck M, et al. A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein. J. Biol. Chem.2008; 283:14257-68.
Background Reference 3:
Ullrich S, Münch A, Neumann S, et al. The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem.2010; 285:20664-74.
Background Reference 4:
Elson GC, de Coignac AB, Aubry JP, et al. BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. Biochem. Biophys. Res. Commun.1999; 264:55-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6063

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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