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Western blot analysis of TMIGD1 in rat liver tissue lysate with TMIGD1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of TMIGD1 in mouse liver tissue with TMIGD1 antibody at 5 μg/mL.
Immunofluorescence of TMIGD1 in mouse liver tissue with TMIGD1 antibody at 20 μg/mL.
Western blot analysis of TMIGD1 in rat liver tissue lysate with TMIGD1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

TMIGD1 Antibody

CATALOG NUMBER: 7615

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (72%)
Immunogen:
TMIGD1 antibody was raised against an 18 amino acid peptide near the center of human TMIGD1.
The immunogen is located within amino acids 150 - 200 of TMIGD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TMIGD1 antibody can be used for detection of TMIGD1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
TMIGD1 antibody is human, mouse and rat reactive. At least two isoforms of TMIGD1 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 29 kDa
Observed: 24 kDa
Purification:
TMIGD1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMIGD1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMIGD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TMIGD1
Additional Names:
TMIGD1 Antibody: TMIGD, UNQ9372, TMIGD, UNQ9372/PRO34164, Transmembrane and immunoglobulin domain-containing protein 1
Protein Accession Number:
NP_996663
Protein Gi Number:
45592949
Ncbi Gene Id Number:
388364
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMIGD1 (transmembrane and immunoglobulin domain containing 1) is a 262 amino acid single-pass membrane protein that contains two Ig-like C2-type (immunoglobulin-like) domains (1,2). It is a nonsecreted, glycosylated protein located in the cytoplasm and cell membrane (1). TMIGD1 might be a possible cell-differentiation marker in the intestine, whose expression is lost during cellular transformation and promote progression of tumorigenesis in the intestinal epithelium (2-4). TMIGD1 has also been postulated to play a role in cell adhesion (2).
Background Reference 1:
Clark HF, Gurney AL, Abaya E, et al. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. Genome Res. 2003; 13:2265-70.
Background Reference 2:
Fujimoto K, Beauchamp RD, Whitehead RH. Identification and isolation of candidate human colonic clonogenic cells based on cell surface integrin expression. Gastroenterology 2002; 123:1941–8.
Background Reference 3:
Yan J, Jiang J, Lim CA, et al. Blimp-1 regulates cell growth through repression of p53 transcription. Proc. Natl. Acad. Sci. USA 2007; 104:1841-6.
Background Reference 4:
Cattaneo E, Laczko E, Buffoli F, et al. Preinvasive colorectal lesion transcriptomes correlate with endoscopic morphology (polypoid vs. nonpolypoid). EMBO Mol Med. 2011; 3:334-47.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7615

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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