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<strong>Figure 1 Western Blot Validation in Human Spleen</strong><br>Loading: 15 μg of lysate.Antibodies: TMIGD2, 7617 1 μg /mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Immunohistochemistry Validation of TMIGD2 in Human Small Intestine Tissue </strong><br> Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TMIGD2 antibody (7617) at 5 μg /ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4° C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Western Blot Validation in Human Spleen
Loading: 15 μg of lysate.Antibodies: TMIGD2, 7617 1 μg /mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

TMIGD2 Antibody

CATALOG NUMBER: 7617

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
TMIGD2 antibody (7617) was raised against a peptide corresponding to 18 amino acids near the carboxy terminus of human TMIGD2.

The immunogen is located within the last 50 amino acids of TMIGD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
TMIGD2 antibody can be used for detection of TMIGD2 by Western blot at 1 - 2 μg /ml. Antibody can also be used for Immunohistochemistry starting at 5 μg /mL

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples. All other applications not yet tested.
Specificity:
TMIGD2 antibody is human reactive. At least two isoforms of TMIGD2 are known to exist; this antibody will detect both isoforms. TMIGD2 antibody is predicted to not cross-react with TMIGD1.
Positive Control 1:
Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 31kD

Observed: 31 kD
Purification:
TMIGD2 antibody is Antigen-affinity purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TMIGD2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TMIGD2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TMIGD2
Additional Names:
TMIGD2 Antibody: IGPR1, IGPR-1, IGPR1, UNQ3059/PRO9879, Transmembrane and immunoglobulin domain-containing protein 2, Immunoglobulin and proline-rich receptor 1
Protein Accession Number:
NP_653216
Protein Gi Number:
281306838
Ncbi Gene Id Number:
126259
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TMIGD2 (transmembrane and immunoglobulin domain containing 1), also known as immunoglobulin-containing and proline-rich receptor 1 (IGPR1), is novel adhesion molecule that is expressed in multiple tissues, primarily in cells of epithelium and endothelium origins (1). TMIGD2 is thought to be involved in angiogenesis and regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. TMIGD2 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration (1).
Background Reference 1:
Rahimi N et al. Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis. Mol. Biol. Cell 2012; 23:1646-56.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7617

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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