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46-503 (0.1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-503 (0.1ug/ml) staining of A431 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TMPRSS3 Antibody

CATALOG NUMBER: 46-503

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-EKIVYHSKYKPKR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in A431 lysates (calculated MW of 49.4kDa according to NP_076927.1). Recommended concentration: 0.1-0.3ug/ml.
Specificity:
This antibody is expected to recognise all four reported isoforms (NP_076927.1; NP_115777.1; NP_115780.1; NP_115781.1)
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TMPRSS3
Additional Names:
transmembrane protease, serine 3, DFNB10, DFNB8, ECHOS1, TADG12, serine protease TADG12
Protein Accession Number:
NP_076927.1,NP_115777.1,NP_115780.1,NP_115781.1
Protein Gi Number:
13173471
Ncbi Gene Id Number:
64699
Background Reference 1:
Lee YJ, Park D, Kim SY, Park WJ. Pathogenic mutations but not polymorphisms in congenital and childhood onset autosomal recessive deafness disrupt the proteolytic activity of TMPRSS3. J Med Genet. 2003 Aug;40(8):629-31. No abstract available.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-503

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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