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To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine TNF-alpha (XP-5285Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-alpha.
This antibody stained colchicine injected mouse brain (hippocampus CA1 region) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
To detect Murine TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Murine TNF-alpha (XP-5285Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine TNF-alpha.

TNF-a Antibody

CATALOG NUMBER: XP-5285

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mTNF-alpha (murine TNF-alpha).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
To detect mTNF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mTNF-alpha.
Sandwich:
To detect mTNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine TNF-α (XP-5285Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mTNF-α.
Western Blot:
To detect mTNF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mTNF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-mTNF alpha specific antibody was purified by affinity chromatography employing immobilized mTNF-alpha matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TNF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Tnf
Additional Names:
DIF, Tnfa, TNF-a, TNFSF2, Tnfsf1a, TNFalpha, TNF-alpha, Tnfsf2, Tumor necrosis factor, Cachectin
Protein Accession Number:
P06804
Protein Gi Number:
135935
Ncbi Gene Id Number:
21926
User Note:
Centrifuge vial prior to opening.
Background:
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5285

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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