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To detect Rat TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat TNF-alpha (XP-5286) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-alpha.
To detect Rat TNF-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Rat TNF-alpha (XP-5286) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Rat TNF-alpha.

TNF-a Antibody (biotin)

CATALOG NUMBER: XP-5286Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Rat
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Rat
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rat TNF-α (rat TNFalpha).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich:
To detect Rat TNF-α by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat TNF-α (XP-5286) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat TNF-α.
Western Blot:
To detect rat TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-rat TNF-α specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
TNF-a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Tnf
Additional Names:
Tnfa, RATTNF, TNF-alpha, Tnfa, Tnfsf2, Tumor necrosis factor, Cachectin, TNF-a
Protein Accession Number:
P16599
Protein Gi Number:
135938
Ncbi Gene Id Number:
24835
User Note:
Centrifuge vial prior to opening.
Background:
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumour bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5286Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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