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Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

TNF alpha Recombinant Protein (HEK293 Expressed)

CATALOG NUMBER: 96-734

Tested Applications:
WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val 77 - Leu 233
Fusion Tag:
Tag free
Tested Applications:
WB
Application Note:
This recombinant protein can be used for WB. For research use only.
Predicted Molecular Weight:
17.4 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized Human TNF-alpha at 5 ug/mL (100 ul/well) can bind Humira with a linear range of 0.04-0.6 ng/mL. The bio-activity was determined by its cytotoxcity assy using L929 Cells with 1ug/ml actinomycin D.
Purity:
>95% as determined by SDS-PAGE.
physical-state:
Lyophilized
Buffer:
PBS, pH7.4
Storage Conditions:
Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
TNF
Additional Names:
DIF, TNF-alpha, TNFA, TNFSF2, cachexin, cachectin, TNF
Protein Accession Number:
P01375
Ncbi Gene Id Number:
7124
Background:
Tumor necrosis factor alpha (TNFα) is a cytokine produced primarily by monocytes and macrophages. It is found in synovial cells and macrophages in the tissues.The primary role of TNFα is in the regulation of immune cells. TNFα is able to induce apoptotic cell death, to induce inflammation, and to inhibit tumorigenesis and viral replication. Dysregulation of TNFα production has been implicated in a variety of human diseases, including major depression, Alzheimer's disease and cancer. Recombinant TNFα is used as an immunostimulant under the INN tasonermin. TNFα can be produced ectopically in the setting of malignancy and parallels parathyroid hormone both in causing secondary hypercalcemia and in the cancers with which excessive production is associated.
Background Reference 1:
Dowlati Y, Herrmann N, et al., 2010, Biol Psychiatry 67 (5): 446–457.
Background Reference 2:
Swardfager W, Lanctot K, et al., 2010, Biol Psychiatry 68 (10): 930–941.
Background Reference 3:
Locksley RM, Killeen N et al., 2001, Cell 104 (4): 487–501.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

96-734

List Size:
0.1 mg

List Price:

$525.00

Datasheet
Shipping Info

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