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TNF-alpha, Soluble Recombinant Protein

CATALOG NUMBER: 90-222

Specifications
source-species:
E. coli
Species:
Human, Mouse
source-species:
E. coli
Recombinant Protein Sequence:
A DDDDK-tag is fused at the N-terminus of human TNF-alpha (aa 85-223).
Fusion Tag:
DDDDK Tag
Application Note:
This recombinant proteins is for research use only.
Biological Activity:
Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
Purity:
>95% (SDS-PAGE).

Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
physical-state:
Lyophilized
Buffer:
Contains PBS. Reconstitute with 50 μl sterile water.PBS containing at least 0.1% BSA should be used for further dilutions.
Concentration:
10 ug size: 0.1 mg/ml after reconstitution.50 ug size: 1 mg/ml after reconstitution.
Storage Conditions:
Stable for at least 1 year after receipt when stored at -20°C.
Ncbi Official Symbol:
TNF
Additional Names:
Tumor Necrosis Factor Ligand Superfamily Member 2, Cachectin
Protein Accession Number:
P01375
Protein Gi Number:
135934
Ncbi Gene Id Number:
7124
Background:
Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (MultimericTNF-alpha (Prod. No. AG-40B-0019). Since the MultimericTNF-alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha can be used.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

90-222

List Size:
50 ug

List Price:

$365.00

Datasheet
Shipping Info

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