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Western blot analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TNFAIP3 in human lung tissue with TNFAIP3 antibody at 5 μg/mL.
Immunofluorescence of TNFAIP3 in Human Lung cells with TNFAIP3 antibody at 20 μg/mL.
Western blot analysis of TNFAIP3 in Jurkat cell lysate with TNFAIP3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TNFAIP3 Antibody

CATALOG NUMBER: 5145

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TNFAIP3 antibody was raised against a 17 amino acid synthetic peptide near the center of human TNFAIP3.
The immunogen is located within amino acids 340 - 390 of TNFAIP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TNFAIP3 antibody can be used for detection of TNFAIP3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 87 kDa
Observed: 95 kDa
Purification:
TNFAIP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TNFAIP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TNFAIP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
Tnfaip3
Additional Names:
TNFAIP3 Antibody: A20, Tnfip3, Tumor necrosis factor alpha-induced protein 3, Putative DNA-binding protein A20, TNF alpha-induced protein 3
Protein Accession Number:
Q60769
Protein Gi Number:
2498097
Ncbi Gene Id Number:
21929
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TNFAIP3 Antibody: TNFAIP3, also known as A20, is located in chromosome band 6q23, a region that is often deleted in B cell lymphomas. Recently, it was identified as a tumor suppressor gene in Hodgkin lymphoma and several subtypes of non-Hodgkin lymphomas. TNFAIP3 was initially identified as a zinc-finger protein that is rapidly and transiently induced by TNF-α, inhibiting NF-κB-dependent gene expression, and protecting cells from TNF-α-cytotoxicity. Overexpression of TNFAIP3 also inhibits the TLR2- and TLR4-mediated interleukin-8 synthesis in airway epithelial cells, suggesting that TNFAIP3 also acts as a negative regulator of TLR-mediated inflammatory responses, thereby protecting the host against harmful over-responses to pathogens. At least two isoforms of TNFAIP3 are known to exist.
Background Reference 1:
Zhang YP, Matthiesen S, Harder R, et al. A 3-cM commonly deleted region in 6q21 in leukemias and lymphomas delineated by fluorescence in situ hybridization. Genes Chromosomes Cancer2000; 27:52-58.
Background Reference 2:
Schmitz R, Hansmann M-L, Bohle V, et al. TNFAIP3 (A20) is a tumor suppressor gene in Hodgkin lymphoma and primary mediastinal B cell lymphoma. J. Exp. Med.2009; 206:981-9.
Background Reference 3:
Honma K, Tsuzuki S, Nakagawa M, et al. TNFAIP3/A20 functions as a novel tumor suppressor gene in several subtypes of non-Hodgkins lymphomas. Blood2009; epub.
Background Reference 4:
Opipari AW Jr, Hu MN, Yabkowitz R, et al. The A20 zinc finger protein protects cells from tumor necrosis factor cytotoxicity. J. Biol. Chem.1992; 267:12424-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5145

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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