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Western blot analysis of extracts of various cell lines, using TNFRSF11B antibody (18-500) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat spleen using TNFRSF11B Antibody (18-500) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using TNFRSF11B Antibody (18-500) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TNFRSF11B Antibody (18-500) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using TNFRSF11B antibody (18-500). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TNFRSF11B antibody (18-500) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

TNFRSF11B Antibody

CATALOG NUMBER: 18-500

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 22-280 of human TNFRSF11B (NP_002537.3).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:10 - 1:100
Positive Control 1:
293T
Positive Control 2:
SW480
Positive Control 3:
MCF7
Positive Control 4:
NCI-H460
Positive Control 5:
K-562
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 44kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TNFRSF11B
Additional Names:
TNFRSF11B, OPG, TR1, OCIF
Ncbi Gene Id Number:
4982
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand, both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest that this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-500

List Size:
100 uL

List Price:

$436.00

Datasheet
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