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Western blot analysis of TNFRSF8-Y479 in mouse liver tissue lysates (35ug/lane)
TNFRSF8-Y479 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TNFRSF8-Y479 Antibody for immunohistochemistry.
Western blot analysis of TNFRSF8-Y479 in mouse liver tissue lysates (35ug/lane)

TNFRSF8-Y479 Antibody

CATALOG NUMBER: 61-707

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This TNFRSF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-487 amino acids from human TNFRSF8.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
64 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNFRSF8
Additional Names:
Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30, CD30, TNFRSF8, CD30, D1S166E
Protein Accession Number:
P28908
Protein Gi Number:
115978
Ncbi Gene Id Number:
943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TNFRSF8 is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity.
Background Reference 1:
Braun, F.K., et al. J. Invest. Dermatol. 130(3):826-840(2010)
Background Reference 2:
Azarpira, N., et al. SaudiKidney Dis Transpl 21(1):31-36(2010)
Background Reference 3:
Edinger, J.T., et al. Am. J. Surg. Pathol. 33(12):1860-1868(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-707

List Size:
400 ul

List Price:

$495.00

Datasheet
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