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42-126 (0.03ug/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-126 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and membrane staining. The nuclear sta
42-126 (0.03ug/ml) staining of Human Cerebellum (A) and Frontal Cortex (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

TNFSF14 Antibody

CATALOG NUMBER: 42-126

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-HEVNPAAHLTGANS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 20-22kDa band observed in Human Cerebellum and Frontal Cortex Brain lysates (calculated MW of 22.4kDa according to NP_742011.1) Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membrane of HeLa cells. Recommended concentration: 10ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003798.2; NP_742011.1).
Positive Control 1:
Cat. No. 1362 - Human Cerebellum, Left Lysate
Cat. No. 1363 - Human Cerebellum, Right Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TNFSF14
Additional Names:
TNFSF14, tumor necrosis factor (ligand) superfamily, member 14, CD258, HVEML, LIGHT, LTg, TR2, delta transmembrane LIGHT, herpesvirus entry mediator A, ligand for herpesvirus entry mediator, tumor necrosis factor ligand superfamily, member 14, tumor necrosis factor receptor-like 2, tumor necrosis factor superfamily member LIGHT
Protein Accession Number:
NP_003798.2,NP_742011.1
Protein Gi Number:
25952144
Ncbi Gene Id Number:
8740
Background Reference 1:
Cohavy O, Zhou J, Ware CF, Targan SR LIGHT is constitutively expressed on T and NK cells in the human gut and can be induced by CD2-mediated signaling J. Immunol. 2005 Jan 174 (2): 646-53

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-126

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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