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Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mL
Immunohistochemistry of TNFSF4 in human spleen tissue with TNFSF4 antibody at 5 μg/ml.
Immunofluorescence of TNFSF4 in human spleen tissue with TNFSF4 antibody at 20 μg/ml.
Western blot analysis of TNFSF4 in rat spleen tissue lysate with TNFSF4 antibody at (A) 0.5 and (B) 1 μg/mL
Trial Size

TNFSF4 Antibody

CATALOG NUMBER: 7243

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (91%)
Immunogen:
Rabbit polyclonal TNFSF4 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human TNFSF4.
The immunogen is located within the last 50 amino acids of TNFSF4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TNFSF4 antibody can be used for detection of TNFSF4 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of TNFSF4 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
20 kDa
Purification:
TNFSF4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TNFSF4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TNFSF4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TNFSF4
Additional Names:
TNFSF4 Antibody: GP34, CD252, OX4OL, TXGP1, CD134L, OX-40L, Tumor necrosis factor ligand superfamily member 4, Glycoprotein Gp34, OX40L
Protein Accession Number:
NP_003317
Protein Gi Number:
4507603
Ncbi Gene Id Number:
7292
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TNFSF4 Antibody: The tumor necrosis factor superfamily member TNFSF4 is a type II membrane bound, non-covalently linked homotrimeric protein. It is expressed on antigen presenting cells, such as dendritic cells and activated B-cells, and also on other cells such as vascular endothelial cells, mast cells, and natural killer cells. TNFSF4 signals specifically through the TNFRSF4 receptor, is expressed predominantly on CD4+T cells but also on certain activated CD8+T cells. TNFRSF4/TNFSF4 functions as a costimulatory signal, which is required for a productive interaction between antigen presenting cells and their target T-cells. It enhances cell proliferation and survival, and increases expression of RANTES, IL-2, IL-3, and IFNγ. TNFRSF4/TNFSF4 signaling plays an important role in immunotolerance.
Background Reference 1:
Arch RH and Thompson CB. 4-1BB and Ox40 are members of a tumor necrosis factor (TNF)-nerve growth factor receptor subfamily that bind TNF receptor-associated factors and activate nuclear factor kappaB. Mol. Cell Biol.1998; 18:558-65.
Background Reference 2:
OX40-OX40 ligand interaction in T-cell-mediated immunity and immunopathy. Adv. Immunol. 2010; 105:63-98

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7243

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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