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Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in IHC on Human Skeletal Muscle at 5.0 ug/ml.

TNNI1 Antibody

CATALOG NUMBER: 29-699

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TNNI1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
TNNI1 antibody can be used for detection of TNNI1 by ELISA at 1:62500. TNNI1 antibody can be used for detection of TNNI1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
21 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store TNNI1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
TNNI1
Additional Names:
TNNI1, DKFZp451O223, TNN1, SSTNI
Protein Accession Number:
NP_003272
Protein Gi Number:
56682969
Ncbi Gene Id Number:
7135
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
Background Reference 1:
Ralli, S., (2005) Am. Heart J. 150 (6), 1220-1227.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-699

List Size:
100 ul

List Price:

$519.00

Datasheet
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