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43-214 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-214 (0.03ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TNNT1 Antibody

CATALOG NUMBER: 43-214

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-EKAQELSDWIHQLE
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 37+35kDa bands observed in Human Skeletal Muscle lysates A single 37kDa band was observed in some lysates.(calculated MW of 32.9kDa according to NP_003274.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03-0.1ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_003274.3; NP_001119604.1; NP_001119605.1).
Predicted Molecular Weight:
Approx 37 + 35kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TNNT1
Additional Names:
TNNT1, troponin T type 1 (skeletal, slow), ANM, FLJ98147, MGC104241, STNT, TNT, TNTS, slow skeletal muscle troponin T, troponin T, slow skeletal muscle, troponin-T1, skeletal, slow
Protein Accession Number:
NP_003274.3,NP_001119604.1,NP_001119605.1
Protein Gi Number:
187173288
Ncbi Gene Id Number:
7138
Background Reference 1:
Midde K, Dumka V, Pinto JR, Muthu P, Marandos P, Gryczynski I, Gryczynski Z, Potter JD, Borejdo J.Myosin cross-bridges do not form precise rigor bonds in hypertrophic heart muscle carrying troponin T mutations.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-214

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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