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Western blot analysis of TNPO3 in rat liver tissue lysate with TNPO3 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of TNPO3 expression in Jurkat cell lysate with TNPO3 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of TNPO3 in rat liver tissue lysate with TNPO3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TNPO3 Antibody

CATALOG NUMBER: 4599

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TNPO3 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human TNPO3.
The immunogen is located within amino acids 220 - 270 of TNPO3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TNPO3 antibody can be used for detection of TNPO3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Positive Control 2:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
Predicted: 95, 101 kDa
Observed: 96 kDa 
Purification:
TNPO3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TNPO3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TNPO3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TNPO3
Additional Names:
TNPO3 Antibody: IPO12, TRNSR, LGMD1F, MTR10A, TRN-SR, TRN-SR2, IPO12, Transportin-3, Importin-12, Imp12
Protein Accession Number:
EAW83708
Protein Gi Number:
119604114
Ncbi Gene Id Number:
23534
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TNPO3 Antibody: TNPO3 is a member of the importin beta/transportin family of nuclear import receptors and is specific for the essential splicing factors termed SR proteins, binding specifically and directly to the arginine/serine (RS)-rich domains of these proteins. Further experiments demonstrated that this interaction occurs only when these RS domains are phosphorylated. More recently, TNPO3 was identified as an HIV dependency factor (HDF) and that depletion of TNPO3 resulted in HIV inhibition after reverse transcription but before integration, suggesting that TNPO3 may be an important drug target in HIV treatment. At least three isoforms of TNPO3 are known to exist.
Background Reference 1:
Kataoka N, Bachorik JL and Dreyfuss G. Transportin-SR, a nuclear import receptor for SR proteins. J. Cell Biol.1999; 145:1145-52.
Background Reference 2:
Lai M-C, Lin R-I, Huang S-Y, et al. A human importin-b family protein, transportin-SR2, interacts with the phosphorylated RS domain of SR proteins. J. Biol. Chem.2000; 275:7950-7.
Background Reference 3:
Brass AL, Dykxhoorn DM, Benita Y, et al. Identification of host proteins required for HIV infection through a functional genomic screen. Science2008; 319:921-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4599

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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