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Western blot analysis of TOCA-1 in mouse brain tissue lysate with in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 1 μg/mL.
Immunohistochemistry of TOCA-1 in human brain tissue with TOCA-1 antibody at 2.5 μg/mL.
Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 μg/mL.
Western blot analysis of TOCA-1 in mouse brain tissue lysate with in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 1 μg/mL.
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TOCA-1 Antibody

CATALOG NUMBER: 4373

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TOCA-1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of the human TOCA-1.
The immunogen is located within the last 50 amino acids of TOCA-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
TOCA-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TOCA-1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOCA-1 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FNBP1L
Additional Names:
TOCA-1 Antibody: TOCA1, C1orf39, TOCA1, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1, Toca-1
Protein Accession Number:
NP_001020119
Protein Gi Number:
68348709
Ncbi Gene Id Number:
54874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TOCA-1 Antibody: Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Background Reference 1:
Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell2004; 118:203-16.
Background Reference 2:
Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem.2006; 281:29042-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4373

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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