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Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 μg/mL.
Immunohistochemical staining of human brain tissue using TOCA-1 antibody at 2.5 μg/mL.
Immunofluorescence of TOCA-1 in Human Brain cells with TOCA-1 antibody at 20 μg/mL.
Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 μg/mL.
Trial Size

TOCA-1 Antibody

CATALOG NUMBER: 4375

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TOCA-1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of the human TOCA-1.
The immunogen is located within amino acids 40 - 90 of TOCA-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Purification:
TOCA-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TOCA-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOCA-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FNBP1L
Additional Names:
TOCA-1 Antibody: TOCA1, C1orf39, TOCA1, Formin-binding protein 1-like, Transducer of Cdc42-dependent actin assembly protein 1, Toca-1
Protein Accession Number:
NP_001020119
Protein Gi Number:
68348709
Ncbi Gene Id Number:
54874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TOCA-1 Antibody: Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Background Reference 1:
Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell2004; 118:203-16.
Background Reference 2:
Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem.2006; 281:29042-43.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4375

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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