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Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of TOLLIP in THP-1 cells with TOLLIP antibody at 2 μg/mL.
Immunofluorescence of TOLLIP in human brain tissue with TOLLIP antibody at 20 μg/ml.<br><br>Green: TOLLIP Antibody (3741) <br> Blue: DAPI staining
Immunohistochemistry of TOLLIP in human brain tissue with TOLLIP antibody at 5 μg/ml.
Western blot analysis of TOLLIP in rat brain cell lysate with TOLLIP antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TOLLIP Antibody

CATALOG NUMBER: 3741

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%), Bovine: (88%)
Immunogen:
TOLLIP antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TOLLIP.
The immunogen is located within the last 50 amino acids of TOLLIP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
TOLLIP antibody can be used for the detection of TOLLIP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Positive Control 2:
Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 3:
Cat. No. 17-008 - THP-1 Cell Slide
Purification:
TOLLIP Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TOLLIP Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOLLIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TOLLIP
Additional Names:
TOLLIP Antibody: IL-1RAcPIP, Toll-interacting protein
Protein Accession Number:
AAH18272
Protein Gi Number:
17390641
Ncbi Gene Id Number:
54472
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TOLLIP Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules to activate various kinases and transcription factors so the organism can respond to potential infection. These adaptor molecules include TOLLIP, MyD88, and TRIF. TOLLIP associates directly with TLR2 and TLR 4, acting as an inhibitor to TLR activation. This negative regulation of TLR signaling may serve to limit the production of proinflammatory mediators during infection and inflammation.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol.2003; 21:335-76.
Background Reference 2:
Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol.2002; 20:197-216.
Background Reference 3:
McGettrick AF and O'Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol. Imm.2004; 41:577-82.
Background Reference 4:
Zhang G and Ghosh S. Negative regulation of Toll-like receptor-mediated signaling by Tollip. J. Biol. Chem.2002; 277:7059-65.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3741

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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