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46-505 (0.03ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-505 (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-505 (0.03ug/ml) staining of A431 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TOM1L2 Antibody

CATALOG NUMBER: 46-505

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog
Immunogen:
The immunogen for this antibody is: CRKKPERSEDALFAL
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 60kDa band observed in Human Fibroblast A431 lysates (calculated MW of 51.8kDa according to NP_653279). Recommended concentration: 0.1-1ug/ml.Immunohistochemistry:In paraffin embedded Human Skeletal Muscle shows variable staining from fibre to fibre. Recommended concentration, 5-10ug/ml.
Specificity:
This antibody is expected to recognise both reported isoforms.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TOM1L2
Additional Names:
TOM1L2, FLJ32746, target of myb1-like 2 (chicken), target of myb1 (chicken) homolog-like 1, target of myb1 (chicken) homolog-like 2, target of myb1-like 2
Protein Accession Number:
NP_001028723.1,NP_001076437.1
Protein Gi Number:
75677323
Ncbi Gene Id Number:
146691
Background Reference 1:
Blagoev B, Ong SE, Kratchmarova I, Mann M. Temporal analysis of phosphotyrosine-dependent signaling networks by quantitative proteomics. Nat Biotechnol. 2004 Sep;22(9):1139-45. Epub 2004 Aug 15.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-505

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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