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Western blot analysis of extracts of various cell lines, using TOP1 antibody (14-483) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse liver using TOP1 antibody (14-483) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug TOP1 antibody (14-483). Western blot was performed from the immunoprecipitate using TOP1 antibody (14-483) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using TOP1 antibody (14-483) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TOP1 Antibody

CATALOG NUMBER: 14-483

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOP1 (NP_003277.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
MCF7
Positive Control 2:
SKOV3
Positive Control 3:
SW480
Positive Control 4:
Jurkat
Positive Control 5:
HeLa
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 120kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TOP1
Additional Names:
TOPI, DNA topoisomerase 1, Scl-70 antigen, topoisomerase (DNA) I, type I DNA topoisomerase
Ncbi Gene Id Number:
7150
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-483

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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