Trial Size

TOPAZ1 Antibody

CATALOG NUMBER: 7737

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%), Sheep: (88%)
Immunogen:
TOPAZ1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TOPAZ1.
The immunogen is located within amino acids 1330 - 1380 of TOPAZ1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TOPAZ1 antibody can be used for detection of TOPAZ1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
TOPAZ1 antibody is human, mouse and rat reactive. At least three isoforms of TOPAZ1 are known to exist.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
Predicted: 65, 186 kDa
Observed: 60, 240 kDa
Purification:
TOPAZ1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TOPAZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOPAZ1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TOPAZ1
Additional Names:
TOPAZ1 Antibody: C3orf77, C3orf77, Testis- and ovary-specific PAZ domain-containing protein 1
Protein Accession Number:
NP_001138502
Protein Gi Number:
222537754
Ncbi Gene Id Number:
375337
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The testis and ovary specific PAZ domain containing 1 (TOPAZ1) protein is a highly conserved protein that is highly expressed in the gonads during germ cell meiosis, suggesting that it may play a role in germ cell development (1). It contains a PAZ domain and a zinc finger domain and is exclusively expressed in adult testis and fetal ovary.
Background Reference 1:
Baillet A, Le Bouffant R, Volff JN, et al. TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates. PLoS One 2011; 6:e26950.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7737

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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