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Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of TOR in L1210 cells with TOR antibody at 2 μg/mL.
Western blot analysis of TOR in L1210 cell lysate with TOR antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TOR Antibody

CATALOG NUMBER: 3485

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
TOR antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human TOR.
The immunogen is located within amino acids 210 - 260 of TOR.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
TOR antibody can be used for the detection of TOR by Western blot at 1 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1284 - L1210 Cell Lysate
Positive Control 2:
Cat. No. 17-204 - L1210 Cell Slide
Purification:
TOR Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TOR Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MTOR
Additional Names:
TOR Antibody: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, FRAP, FK506-binding protein 12-rapamycin complex-associated protein 1, mTOR
Protein Accession Number:
NP_004949
Protein Gi Number:
4826730
Ncbi Gene Id Number:
2475
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TOR Antibody: The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. It was initially discovered as a kinase whose ability to stimulate T cell proliferation in response to IL-2 could be inhibited by the immunosuppressive drug rapamycin. Rapamycin inhibits TOR in other cell types resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation. TOR is normally associated with the regulatory proteins RAPTOR and GbetaL. Its downstream targets are thought to be the ribosomal protein S6 kinases and the eukaryotic initiation factor 4E binding proteins (4EBPs). Regulation of these protein families allows TOR to control protein biosynthesis.
Background Reference 1:
Shamji AF, Ngheim P, and Schreiber SL. Integration of growth factor and nutrient signaling: implications for cancer biology. Mol. Cell 2003; 12:271-80.
Background Reference 2:
Sabatini DM, Erdjument-Bromage H, Lui M, et al. RAFT1: a mammalian protein that binds to FKP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 1994; 78:35-43.
Background Reference 3:
Cardenas ME, Zhu D, and Heitman J. Molecular mechanisms of immunosuppression by cyclosporine, FK506, and rapamycin. Curr. Opin. Nephrol. Hypertens. 1995; 4:472-7.
Background Reference 4:
Fingar DC and Blenis J. Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression. Oncogene 2004; 23:3151-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3485

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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