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Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of TOSO in K562 cell lysate with TOSO antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Toso Antibody

CATALOG NUMBER: 2273

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Toso antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human Toso.
The immunogen is located within the first 50 amino acids of Toso.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Toso antibody can be used for detection of Toso by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
Predicted: 43 kDa
Observed: 41 kDa
Purification:
Toso Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Toso Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
Toso antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FAIM3
Additional Names:
Toso Antibody: FCMR, TOSO, Fas apoptotic inhibitory molecule 3, Regulator of Fas-induced apoptosis Toso
Protein Accession Number:
NP_005440
Protein Gi Number:
4885641
Ncbi Gene Id Number:
9214
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Toso Antibody: Apoptosis is an important process by which normal tissue homeostasis and function are maintained. One of the major signals that regulate this process is mediated by the activation of the Fas receptor by its ligand. This leads to the formation of a Fas-associated death domain (FADD)- containing death-inducing signaling complex and the activation of caspase-8, which in turn activates downstream effector caspases, such as caspase-3 and -7. Recent experiments have shown that overexpression of Toso, a novel regulator of Fas-induced apoptosis in lymphoid cells, in Jurkat cells as well as transgenic mice render these cells resistant to Fas-induced apoptosis but not to TRAIL-induced apoptosis. Furthermore, Toso was found to associate with FADD, suggesting that Toso functions by disrupting the formation of the death-inducing signaling complex.
Background Reference 1:
Curtin JF and Cotter TG. Live and let die: regulatory mechanisms in Fas-mediated apoptosis. Cell Signal. 2003; 15:983-92.
Background Reference 2:
Hitoshi Y, Lorens J, Kitada S-I, et al. Toso, a cell surface, specific regulator of Fas-induced apoptosis in T cells. Immunity 1998; 8:461-71.
Background Reference 3:
Song Y and Jacob CO. The mouse cell surface protein Toso regulates Fas/Fas ligand-induced apoptosis through its binding to Fas-associated death domain. J. Biol. Chem.2005; 280:9618-26.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2273

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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