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Western blot analysis of TOX in human colon tissue lysate with TOX antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of TOX in human colon tissue with TOX antibody at 20 μg/mL.
Western blot analysis of TOX in human colon tissue lysate with TOX antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TOX Antibody

CATALOG NUMBER: 6987

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (76%)
Immunogen:
TOX antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TOX.
The immunogen is located within amino acids 130 - 180 of TOX.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TOX antibody can be used for detection of TOX by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
No alternatively spliced transcript variants have been observed.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
58 kDa
Purification:
TOX Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TOX Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TOX antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TOX
Additional Names:
TOX Antibody: TOX1, KIAA0808, Thymocyte selection-associated high mobility group box protein TOX, Thymus high mobility group box protein TOX
Protein Accession Number:
NP_055544
Protein Gi Number:
7662322
Ncbi Gene Id Number:
9760
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TOX Antibody: TOX (thymocyte selection-associated high mobility group (HMG) box protein) is a member of the HMG box family of DNA-binding proteins and likely plays a role in the regulation of T-cell development. It is a 526 amino acid nuclear protein and the expression of TOX is upregulated by pre-T cell receptor (pre-TCR) and TCR activation in immature thymocytes. TOX-mediated positive selection is associated with up-regulation of RUNX3 and is calcineurin dependent. TOX-dependent transition to the CD4+CD8 stage is required for development of class II major histocompatibility complex-specific T cells.
Background Reference 1:
Wilkinson B, Chen JY, Han P, et al. TOX: an HMG box protein implicated in the regulation of thymocyte selection. Nat. Immunol. 2002; 3:272-80.
Background Reference 2:
Saito T and Watanabe N. Positive and negative thymocyte selection. Crit. Rev. Immunol. 1998; 18:359-70
Background Reference 3:
Aliahmad P, O'Flaherty E, Han P, et al. TOX provides a link between calcineurin activation and CD8 lineage commitment. J. Exp. Med. 2004; 199:1089-99.
Background Reference 4:
Aliahmad P, Kadavallore A, de la Torre B, et al. TOX is required for development of the CD4 T cell lineage gene program. J. Immunol. 2011; 187:5931-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6987

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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