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Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Western Blot at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.

TP53RK Antibody

CATALOG NUMBER: 58-875

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TP53RK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human TP53RK.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TP53RK
Additional Names:
TP53-regulating kinase, Atypical serine/threonine protein kinase TP53RK, EKC/KEOPS complex subunit TP53RK, 36--, Nori-2, p53-related protein kinase, TP53RK, C20orf64, PRPK
Protein Accession Number:
Q96S44
Protein Gi Number:
26398348
Ncbi Gene Id Number:
112858
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Background Reference 1:
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Background Reference 2:
Facchin, S., et al. Cell. Mol. Life Sci. 64 (19-20), 2680-2689 (2007) :
Background Reference 3:
Abe, Y., et al. Biochem. Biophys. Res. Commun. 344(1):377-385(2006)
Background Reference 4:
Facchin, S., et al. FEBS Lett. 549 (1-3), 63-66 (2003) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-875

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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