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Western Blot at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue with TPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of widr cells using TPH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of TPH1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.

TPH1 Antibody

CATALOG NUMBER: 62-545

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Rabbit, Rat, Xenopus
Immunogen:
This TPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human TPH1.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:500
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TPH1
Additional Names:
Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH
Protein Accession Number:
P17752
Protein Gi Number:
116242823
Ncbi Gene Id Number:
7166
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter.
Background Reference 1:
Johansson,T.A., et.al., BMC Cancer 9, 321 (2009)
Background Reference 2:
Kuhn,D.M., et.al., J. Neurochem. 68 (5), 2220-2223 (1997)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-545

List Size:
400 ul

List Price:

$495.00

Datasheet
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