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Western blot analysis in U251,A549,Jurkat cell line lysates (35ug/lane).
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in U251,A549,Jurkat cell line lysates (35ug/lane).

TPM4 Antibody

CATALOG NUMBER: 56-627

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This TPM4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human TPM4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TPM4
Additional Names:
Tropomyosin alpha-4 chain, TM30p1, Tropomyosin-4, TPM4
Protein Accession Number:
P67936
Protein Gi Number:
54039751
Ncbi Gene Id Number:
7171
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the tropomyosin family ofactin-binding proteins involved in the contractile system ofstriated and smooth muscles and the cytoskeleton of non-musclecells. Tropomyosins are dimers of coiled-coil proteins thatpolymerize end-to-end along the major groove in most actinfilaments. They provide stability to the filaments and regulateaccess of other actin-binding proteins. In muscle cells, theyregulate muscle contraction by controlling the binding of myosinheads to the actin filament. Multiple transcript variants encodingdifferent isoforms have been found for this gene. [provided byRefSeq].
Background Reference 1:
Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010)
Background Reference 2:
Vlahovich, N., et al. Cell Motil. Cytoskeleton 65(1):73-85(2008)
Background Reference 3:
Montesano Gesualdi, N., et al. Free Radic. Res. 40(5):467-476(2006)
Background Reference 4:
Hossain, M.M., et al. J. Biol. Chem. 280(51):42442-42453(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-627

List Size:
400 ul

List Price:

$495.00

Datasheet
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