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(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.
TPPP3 Antibody IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
(LEFT)Western blot analysis of TPPP3 Antibody in WiDr cell tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of TPPP3 Antibody in mouse cerebellum tissue lysates (35ug/lane).TPPP3 (arrow) was detected using the purified Pab.

TPPP3 Antibody

CATALOG NUMBER: 61-751

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This TPPP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-146 amino acids from the C-terminal region of human TPPP3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
19 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TPPP3
Additional Names:
Tubulin polymerization-promoting protein family member 3, TPPP/p20, TPPP3
Protein Accession Number:
Q9BW30
Protein Gi Number:
28380040
Ncbi Gene Id Number:
51673
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TPPP3 binds tubulin and has microtubule bundling activity. TPPP3 may play a role in cell proliferation and mitosis.
Background Reference 1:
Zhou, W., et al. Mol. Cell. Biochem. 333 (1-2), 91-98 (2010) Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009) Vincze, O., et al. Biochemistry 45(46):13818-13826(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-751

List Size:
400 ul

List Price:

$495.00

Datasheet
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