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46-508 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-508 (2.5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
46-508 (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

TPTE Antibody

CATALOG NUMBER: 46-508

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: C-ERRTDKTHSEKFQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50-55kDa band observed in Human Testis lysates (calculated MW of 60.2kDa according to NP_954869.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 2.5ug/ml.
Specificity:
This antibody is expected to recognise all reported isoforms (NP_954870.2, NP_954868.1 and NP_954869.1). It may cross-react with the almost identical TPTE2 (GeneID 93492; NP_570141.2, NP_954864.1, NP_954863.1).
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
TPTE
Additional Names:
TPTE, PTEN2 , transmembrane phosphatase with tensin homology , tensin, putative protein-tyrosine phosphatase
Protein Accession Number:
NP_954870.2,NP_954868.1,NP_954869.1
Protein Gi Number:
109689707
Ncbi Gene Id Number:
7179
Background Reference 1:
Tapparel C, Reymond A, Girardet C, Guillou L, Lyle R, Lamon C, Hutter P, Antonarakis SE. The TPTE gene family: cellular expression, subcellular localization and alternative splicing. Gene. 2003 Dec 24;323:189-99.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-508

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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