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Western blot analysis in Hela cell line lysates (35ug/lane).
TRADD Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of TRADD Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot analysis in Hela cell line lysates (35ug/lane).

TRADD Antibody

CATALOG NUMBER: 56-146

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TRADD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 126-151 amino acids from the Central region of human TRADD.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRADD
Additional Names:
Tumor necrosis factor receptor type 1-associated DEATH domain protein, TNFR1-associated DEATH domain protein, TNFRSF1A-associated via death domain, TRADD
Protein Accession Number:
Q15628
Protein Gi Number:
6094511
Ncbi Gene Id Number:
8717
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a death domaincontaining adaptor molecule that interacts with TNFRSF1A/TNFR1 andmediates programmed cell death signaling and NF-kappaB activation.This protein binds adaptor protein TRAF2, reduces the recruitmentof inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thussuppresses TRAF2 mediated apoptosis. This protein can also interactwith receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and isinvolved in the Fas-induced cell death pathway. [provided byRefSeq].
Background Reference 1:
Pointon, J.J., et al. Ann. Rheum. Dis. 69(6):1243-1246(2010)
Background Reference 2:
Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009)
Background Reference 3:
Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
Background Reference 4:
Liang, X.S., et al. Br. J. Haematol. 146(4):418-423(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-146

List Size:
400 ul

List Price:

$495.00

Datasheet
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