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Western blot analysis of extracts of various cell lines, using TRAF3 antibody (18-938) at 1:400 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using TRAF3 antibody (18-938). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using TRAF3 antibody (18-938) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TRAF3 Antibody

CATALOG NUMBER: 18-938

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide of human TRAF3
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Positive Control 1:
22Rv1
Positive Control 2:
SW480
Positive Control 3:
MCF7
Positive Control 4:
U-937
Positive Control 5:
ES-2
Positive Control 6:
Raji
Predicted Molecular Weight:
Observed: 70kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TRAF3
Additional Names:
TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, CD40bp, CAP1, TNF receptor-associated factor 3
Ncbi Gene Id Number:
7187
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

18-938

List Size:
100 uL

List Price:

$436.00

Datasheet
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