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Western blot analysis of TRAF3 in 3T3 cell lysate with TRAF3 antibody at (A) 1, and (B) 2 μg/mL.
Immunofluorescence of TRAF3 in 3T3 cells with TRAF3 antibody at 2 μg/mL.
Western blot analysis of TRAF3 in 3T3 cell lysate with TRAF3 antibody at (A) 1, and (B) 2 μg/mL.
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TRAF3 Antibody

CATALOG NUMBER: 3599

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TRAF3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TRAF3.
The immunogen is located within amino acids 410 - 460 of TRAF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TRAF3 antibody can be used for the detection of TRAF3 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 2 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
TRAF3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TRAF3 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRAF3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRAF3
Additional Names:
TRAF3 Antibody: CAP1, LAP1, CAP-1, CRAF1, IIAE5, CD40bp, CAP1, TNF receptor-associated factor 3
Protein Accession Number:
NP_663777
Protein Gi Number:
22027618
Ncbi Gene Id Number:
7187
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRAF3 Antibody: Tumor necrosis factor (TNF) receptor associated factors (TRAFs) are the major signal transducers for the TNF receptor superfamily and the interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. TRAF3 was first identified by its interaction with CD40 and the Epstein-Barr virus transforming protein LMP1. Several TRAF3 mRNA splice variants exist and some of these can activate the transcription factor NF-κB. Besides CD40, TRAF3 also interacts with the TRFR superfamily member lymphotoxin-beta receptor (LTbetaR) in association with TRAF2 and the apoptosis inhibitors cIAP1 and Smac. It has been suggested that TRAF3 induces mitochondria-mediated apoptosis upon binding of the TNF family cytokine LIGHT by LTbetaR.
Background Reference 1:
Arch RH, Gedrich RW, and Thompson CB. Tumor necrosis factor receptor-associated factors (TRAFs) - a family of adaptor proteins that regulate life and death. Genes Dev. 1998; 12:2821-30.
Background Reference 2:
Cheng G, Cleary AM, Ye Z, et al. Involvement of CRAF1, a relative of TRAF, in CD40 signaling. Science 1995; 267:1494-8.
Background Reference 3:
Mosialos G, Birkenbach M, Yalamanchili R, et al. The Epstein-Barr virus transforming protein LMP1 engages signaling proteins for the tumor necrosis factor receptor family. Cell 1995; 80:389-99.
Background Reference 4:
van Eyndhoven WG, Gamper CJ, Cho E, et al. TRAF-3 mRNA splice-deletion variants encode isoforms that induce NF0κB activation. Mol. Immunol. 1999; 36:647-58.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3599

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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