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Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 μg/mL.
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.
Trial Size

TRAF6 Antibody

CATALOG NUMBER: 3129

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (92%)
Immunogen:
TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6.
The immunogen is located within the last 50 amino acids of TRAF6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 3:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
TRAF6 Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRAF6 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRAF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRAF6
Additional Names:
TRAF6 Antibody: RNF85, MGC:3310, RNF85, E3 ubiquitin-protein ligase TRAF6
Protein Accession Number:
NP_004611
Protein Gi Number:
4759254
Ncbi Gene Id Number:
7189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.
Background Reference 3:
Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.
Background Reference 4:
Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3129

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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