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Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.
Immunocytochemistry of TRAF6 in K562 cells with TRAF6 antibody at 0.5 μg/mL.
Immunofluorescence of TRAF6 in K562 cells with TRAF6 antibody at 20 μg/mL.
Western blot analysis of TRAF6 in PC-3 cell lysates with TRAF6 antibody at 1 μg/mL in the presence (A) or absence (B) of 1 μg blocking peptide.
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TRAF6 Antibody

CATALOG NUMBER: 3129

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (86%), Pig: (92%)
Immunogen:
TRAF6 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human TRAF6.
The immunogen is located within the last 50 amino acids of TRAF6.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TRAF6 antibody can be used for detection of TRAF6 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 3:
Cat. No. 17-004 - K-562 Cell Slide
Purification:
TRAF6 Antibody is Ion exchange chromatography purified.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
TRAF6 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRAF6 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRAF6
Additional Names:
TRAF6 Antibody: RNF85, MGC:3310, RNF85, E3 ubiquitin-protein ligase TRAF6
Protein Accession Number:
NP_004611
Protein Gi Number:
4759254
Ncbi Gene Id Number:
7189
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRAF6 Antibody: Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK.
Background Reference 1:
Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76.
Background Reference 2:
Wu H. Assembly of post-receptor signaling complexes for the tumor necrosis factor superfamily. Adv. Protein Chem. 2004; 68:225-79.
Background Reference 3:
Wajant H and Scheurich P. Analogies between Drosophila and mammalian TRAF pathways. Prog. Mol. Subcell. Biol. 2004; 34:47-72.
Background Reference 4:
Kobayashi T, Walsh PT, Walsh MC, et al. TRAF6 is a critical factor for dendritic cell maturation and development. Immunity 2003; 19:353-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

3129

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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