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43-405 (0.3ug/ml) staining of A43 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-405 (0.003ug/ml) staining of Pig Brain (A) and Heart (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
43-405 (0.3ug/ml) staining of A43 (A) and HeLa (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Transcription factor E2F4 Antibody

CATALOG NUMBER: 43-405

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-PKELSEIFDPTR
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 50kDa band observed in lysates of cell lines A431 and HeLa (calculated MW of 44.0kDa according to NP_001941.2). Recommended concentration: 0.1-0.3ug/ml.Approx 40+50kDa bands observed in Pig Brain and Heart lysates (calculated MW of 43.2kDa according to Pig XP_003126981.3). Recommended concentration: 0.01-0.003ug/ml.
Predicted Molecular Weight:
Approx 50kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Transcription factor E2F4
Additional Names:
E2F4, E2F transcription factor 4, p107/p130-binding, E2F-4, p107/p130-binding protein, transcription factor E2F4
Protein Accession Number:
NP_001941.2
Protein Gi Number:
12669915
Ncbi Gene Id Number:
1874
Background Reference 1:
Beshiri ML, Holmes KB, Richter WF, Hess S, Islam AB, Yan Q, Plante L, Litovchick L, Gévry N, Lopez-Bigas N, Kaelin WG, Benevolenskaya EV.Coordinated repression of cell cycle genes by KDM5A and E2F4 during differentiation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-405

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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