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Western blot analysis of lysates from Hep G2 cells using Transferrin Rabbit mAb (A19130) at 1:1000 dilution incubated overnight at 4℃. Hep G2 cells were treated with BFA (30 ng/mL) at 37℃ for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded Human placenta using Transferrin Rabbit mAb (A19130) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Western blot analysis of lysates from Hep G2 cells using Transferrin Rabbit mAb (A19130) at 1:1000 dilution incubated overnight at 4℃. Hep G2 cells were treated with BFA (30 ng/mL) at 37℃ for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3 % nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 180s.

Transferrin Rabbit Recombinant Antibody

CATALOG NUMBER: 81-396

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
WB,1:1000 - 1:2000, IHC-P,1:100 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Hep G2 treated with BFA
Predicted Molecular Weight:
77kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TF
Additional Names:
TFQTL1; PRO1557; PRO2086; HEL-S-71p; Transferrin
Protein Accession Number:
P02787
Ncbi Gene Id Number:
7018
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-396

List Size:
100 uL

List Price:

$477.00

Datasheet
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