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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
TRAP1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

TRAP1 Antibody

CATALOG NUMBER: 57-499

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This TRAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-570 amino acids from the C-terminal region of human TRAP1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
80 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRAP1
Additional Names:
Heat shock protein 75 kDa, mitochondrial, HSP 75, TNFR-associated protein 1, Tumor necrosis factor type 1 receptor-associated protein, TRAP-1, TRAP1, HSP75
Protein Accession Number:
Q12931
Protein Gi Number:
67477458
Ncbi Gene Id Number:
10131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HSP90 proteins are highly conserved molecular chaperonesthat have key roles in signal transduction, protein folding,protein degradation, and morphologic evolution. HSP90 proteinsnormally associate with other cochaperones and play important rolesin folding newly synthesized proteins or stabilizing and refoldingdenatured proteins after stress. TRAP1 is a mitochondrial HSP90protein. Other HSP90 proteins are found in cytosol (see HSP90AA1;MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chenet al., 2005 [PubMed 16269234]).
Background Reference 1:
Liu, D., et al. Cancer Lett. 296(2):194-205(2010)
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Nguyen, M.C., et al. Cancer Immunol. Immunother. 59(9):1313-1323(2010)
Background Reference 4:
Landriscina, M., et al. Cancer Res. 70(16):6577-6586(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-499

List Size:
400 ul

List Price:

$495.00

Datasheet
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