TRAP2 Antibody

CATALOG NUMBER: 46-512

Clonality:
Polyclonal
Tested Applications:
ELISA, Flow, ICC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-VILRKNPNYDL
Conjugate:
Unconjugated
Tested Applications:
ELISA, Flow, ICC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 100kDa band observed in lysates of cell lines HeLa, U251, NIH3T3 and KNRK (calculated MW of 100kDa according to Human NP_002799.3, Mouse NP_598862.1 and Rat NP_001026809.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PSMD2
Additional Names:
PSMD2, TRAP2, MGC14274, S2, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, 55.11 protein, TNFR-associated protein 2, 26S proteasome subunit p97, 26S proteasome regulatory subunit S2, 26S proteasome non-ATPase regulatory subunit 2, tumor necrosis factor receptor-associated protein 2, Rpn1, proteasome 26S non-ATPase subunit 2
Protein Accession Number:
NP_002799.3
Protein Gi Number:
25777602
Ncbi Gene Id Number:
5708
Background Reference 1:
Kuai J, Wooters J, Hall JP, Rao VR, Nickbarg E, Li B, Chatterjee-Kishore M, Qiu Y, Lin LL. NAK is recruited to the TNFR1 complex in a TNFalpha-dependent manner and mediates the production of RANTES: identification of endogenous TNFR-interacting proteins by a proteomic approach. J Biol Chem. 2004 Dec 17;279(51):53266-71. Epub 2004 Oct 13.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-512

List Size:
0.1 mg

List Price:

$495.00

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