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Western blot analysis of TRB3 in mouse kidney tissue lysate with TRB3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of TRB3 in mouse liver tissue with TRB3 antibody at 5 μg/mL.
Immunofluorescence of TRB3 in mouse liver tissue with TRB3 antibody at 20 μg/mL.
Western blot analysis of TRB3 in mouse kidney tissue lysate with TRB3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

TRB3 Antibody

CATALOG NUMBER: 8099

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (76%)
Immunogen:
TRB3 antibody was raised against a 17 amino acid peptide near the center of human TRB3.
The immunogen is located within amino acids 100 - 150 of TRB3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRB3 antibody can be used for detection of TRB3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
TRB3 antibody is human, mouse and rat reactive. At least two isoforms of TRB3 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 39 kDa
Observed: 39 kDa
Purification:
TRB3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRB3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRB3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TRB3
Additional Names:
Tribbles homolog 3, tribbles pseudokinase 3, TRIB3, NIPK, SINK, SKIP3, C20orf97
Protein Accession Number:
NP_066981
Protein Gi Number:
31542265
Ncbi Gene Id Number:
57761
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRB3 was initially identified as a novel kinase-like gene that was overexpressed in multiple human tumors and regulated by hypoxia (1). In the liver, TRB3 binds and inhibits AKT activity, leading to impaired insulin signaling (2), whereas in adipose tissue TRB3 is involved in the regulation of fatty acid oxidation through ubiquitination of Acetyl-CoA carboxylase (3). Endoplasmic reticulum (ER) stress induces TRB3 expression in skeletal muscle, leading to a decrease in insulin signaling and glucose uptake, suggesting that inhibition of TRB3 expression may be a potential therapeutic treatment for managing insulin resistance (4).
Background Reference 1:
Bowers AJ, Scully S, and Boylan JF. SKIP3, a novel drosophila tribbles ortholog, is overexpressed in human tumors and is regulated by hypoxia. Oncogene 2003; 22:2823-35.
Background Reference 2:
Du K, Herzig S, Kulkarni RN, et al. TRB3: a tribbles homolog that inhibits Akt/PKB activation by insulin in liver. Science 2003; 300:1574-7.
Background Reference 3:
Qi L, Heredia JE, Altarejos JY, et al. TRB3 inks the E3 ubiquitin ligase COP1 to lipid metabolism. Science 2006; 312:1763-6.
Background Reference 4:
Koh HJ, Toyoda T, Didesch MM, et al. Tribbles 3 mediates endoplasmic reticulum stress-induced insulin resistance in skeletal muscle. Nat. Commun. 2013; 4:1871.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8099

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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