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Western blot analysis of extracts of Mouse brain, using TREM2 antibody (13-679) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-251MG cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Mouse brain, using TREM2 antibody (13-679) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

TREM2 Antibody

CATALOG NUMBER: 13-679

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human TREM2 (NP_061838.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
Positive Control 1:
Mouse brain
Predicted Molecular Weight:
Observed: 25kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TREM2
Additional Names:
TREM2, triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, OTTHUMP00000039771, triggering receptor expressed on myeloid cells 2a
Ncbi Gene Id Number:
54209
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-679

List Size:
100 uL

List Price:

$436.00

Datasheet
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