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42-134 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain
42-134 Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain

Trem2 Antibody

CATALOG NUMBER: 42-134

Clonality:
Polyclonal
Tested Applications:
ELISA, IF
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat
Immunogen:
The immunogen for this antibody is: C-QVEHSTSRNQET
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments showed a band at approx 26kDa in Mouse and Rat Adrenal gland lysates after 1ug/ml antibody staining (calculated MW of 24.5kDa according to Mouse NP_112544.1 and 24.9 according to Rat NP_001100354.1). Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the Plasma Membrane of NIH3T3-L1 (adipocyte;like) cells. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Trem2
Additional Names:
Trem2, triggering receptor expressed on myeloid cells 2 , Trem2a, Trem2b, Trem2c , triggering receptor expressed on myeloid cells 2a, triggering receptor expressed on myeloid cells 2b, triggering receptor expressed on myeloid cells 2c
Protein Accession Number:
NP_112544.1
Protein Gi Number:
13994125
Ncbi Gene Id Number:
83433(mouse);301227(rat);
Background Reference 1:
Takahashi K, Prinz M, Stagi M, Chechneva O, Neumann H. TREM2-transduced myeloid precursors mediate nervous tissue debris clearance and facilitate recovery in an animal model of multiple sclerosis. PLoS Med. 2007 Apr;4(4):e124.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-134

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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