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Western blot analysis of TREX1 in human spleen tissue lysate with TREX1 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 μg/mL.
Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 μgg/mL.
Western blot analysis of TREX1 in human spleen tissue lysate with TREX1 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TREX1 Antibody

CATALOG NUMBER: 4969

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%)
Immunogen:
TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1.
The immunogen is located within amino acids 220 - 270 of TREX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TREX1 antibody will not cross-react with the related protein TREX2. At least three isoforms of TREX1 are known to exist; this antibody will recognize all three isoforms.
Positive Control 1:
Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 33, 35, 41 kDa
Observed: 31 kDa
Purification:
TREX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TREX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TREX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TREX1
Additional Names:
TREX1 Antibody: CRV, AGS1, DRN3, HERNS, Three prime repair exonuclease 1, DNase III
Protein Accession Number:
NP_057465
Protein Gi Number:
7705353
Ncbi Gene Id Number:
11277
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TREX1 Antibody: Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.
Background Reference 1:
Mazur DJ and Perrino FW. Identification and expression of the TREX1 and TREX2 cDNA sequences encoding mammalian 3→5 exonucleases. J. Biol. Chem. 1999; 274:19655-60.
Background Reference 2:
Cortez D, Guntuku S, Qin J, et. al. ATR and ATRIP: partners in checkpoint signaling. Science 2001 294:1713-6
Background Reference 3:
Stetson DB, Ko JS, Heidmann T, et al. Trex1 prevents cell-intrinsic initiation of autoimmunity. Cell 2008 134:587-98.
Background Reference 4:
Mazur DJ and Perrino FW. Structure and expression of the TREX1 and TREX2 3'→5' exonuclease genes. J. Biol. Chem. 2001; 276:14718-27.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

4969

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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