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Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-TRIB1 Pab (AP7726b) in T47D cell line lysate (35ug/lane).
Western Blot at 1:2000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

TRIB1 Antibody

CATALOG NUMBER: 63-171

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TRIB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 341-370 amino acids from the C-terminal region of human TRIB1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIB1
Additional Names:
Tribbles homolog 1, TRB-1, G-protein-coupled receptor-induced gene 2 protein, GIG-2, SKIP1, TRIB1 (HGNC:16891)
Protein Accession Number:
Q96RU8
Protein Gi Number:
83305929
Ncbi Gene Id Number:
10221
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIB1 antibody, also called gprotein coupled receptor induced, skip1, gig2, c8fwATP, functions in binding protein binding, protein kinase inhibitor activity, and protein serine/threonine kinase activities.Overexpression of TRIB1 in HeLa cells repressed the basal activity of the IL8 promoter by inhibiting AP1 activity. Overexpression of TRIB1 inhibited oncogenic Ras -driven AP1 activation and MEKK1-mediated AP1 activation. ERK activation was enhanced by TRIB1. Coimmunoprecipitation and yeast 2-hybrid assays showed that MEK1 interacted with both TRIB1 and TRIB3, and MKK4 interacted specifically with TRIB1. Cotransfection of MKK4 enhanced the level of TRIB1, indicating that the TRIB-MAPKK interaction stabilized TRIB1.
Background Reference 1:
Kiss-Toth, E., et al., J. Biol. Chem. 279(41):42703-42708 (2004).
Background Reference 2:
Wu, M., et al., J. Biol. Chem. 278(29):27072-27079 (2003).
Background Reference 3:
Wilkin, F., et al., Eur. J. Biochem. 248(3):660-668 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-171

List Size:
400 ul

List Price:

$495.00

Datasheet
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